Journal: bioRxiv

Article Title: Magnetoelectric nanodiscs diminish motor deficits in a model of Parkinson’s disease

doi: 10.1101/2025.06.04.657885

Figure Lengend Snippet: ( A) Schematic illustration of the MEND-mediated treatment on neurodegeneration. ( B ) EDS mapping of electron diffraction spectroscopy for Fe, Co, Ti, and Ba on ( C ) High-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) image of MENDs. Scale bars indicate 100 nm. ( D ) SEM image of neurons decorated with MENDs, overlayed with the EDS mapping. Scale bar is 1 μ m. ( E ) Potential changes in the electrochemical cell, including the electric polarization generated by MENDs under magnetic field application on the working electrode. ( F ) Average and ( G ) individual traces of GCaMP6s relative fluorescence changes on cultured hippocampal neurons. Grey shade areas indicate the magnetic field (MF) On. (F) Line and shaded area represent mean and standard error, respectively. ( H ) Schematic illustration for MEND injection in left STN and PBS injection in right STN. ( I ) Quantification of c-Fos expression from the inmages of ( J ) left and ( K ) right STN after the exposure the magnetic field. The statistical analysis was performed with paired t-test; p = 0.0084, t = 6.21, DF = 3.

Article Snippet: Four days after seeding, the neurons were transduced with 1 μl of an adeno-associated virus serotype 9 (AAV9) carrying a fluorescent calcium ion indicator GCaMP6s (AAV9-hSyn::GCaMP6s, Addgene viral prep #100843-AAV9, >1 × 10 13 IU ml −1 ).

Techniques: Spectroscopy, Transmission Assay, Electron Microscopy, Generated, Fluorescence, Cell Culture, Injection, Expressing